Panteley Denev
University of Food Technologies - Plovdiv, Organic chemistry, Faculty Member
Ficus carica L. belongs to the family Moraceae. It is commonly known as fig, which is a medium sized deciduous tree widely distributed in sub-tropical and tropical countries. It has been used as fruit and medicine for several centuries.... more
Ficus carica L. belongs to the family Moraceae. It is commonly known as fig, which is a medium sized
deciduous tree widely distributed in sub-tropical and tropical countries. It has been used as fruit and medicine
for several centuries. Phytoconstituents like flavonoids, phenolics, fatty acis, proanthocyanidine, anthocyanins,
phytosteroles (campesterol, stigmasterol, sitosterol), xanthotoxin, psoralens, bergapten, xanthotoxol have been
extracted from leaves and fruits, and peptides from latex. Traditionally the fruit extracts possessed
hypoglycemic, diuretic, expectorant, antioxidant, immunity, anthelmintic, hepetoprotective activities, latex has
anthelmintic and anticarcinogenic. The present review is therefore, an effort to give a detailed survey of the
literature on its pharmacognosy, phytochemistry, and pharmacological properties.
deciduous tree widely distributed in sub-tropical and tropical countries. It has been used as fruit and medicine
for several centuries. Phytoconstituents like flavonoids, phenolics, fatty acis, proanthocyanidine, anthocyanins,
phytosteroles (campesterol, stigmasterol, sitosterol), xanthotoxin, psoralens, bergapten, xanthotoxol have been
extracted from leaves and fruits, and peptides from latex. Traditionally the fruit extracts possessed
hypoglycemic, diuretic, expectorant, antioxidant, immunity, anthelmintic, hepetoprotective activities, latex has
anthelmintic and anticarcinogenic. The present review is therefore, an effort to give a detailed survey of the
literature on its pharmacognosy, phytochemistry, and pharmacological properties.
Research Interests: Fig and Phytonutrients
The content of polyphenols in Fumaria officinalis L. and Fumaria thuretii Bioss. from two different populations, naturally grown in Bulgaria has been studied. Significant differences between concentrations of main polyphenols (rutin,... more
The content of polyphenols in Fumaria officinalis L. and Fumaria thuretii Bioss. from two different
populations, naturally grown in Bulgaria has been studied. Significant differences between concentrations of
main polyphenols (rutin, hyperoside and syringic acid) and antioxidant activity in two different populations of
both analyzed species were not established (RSD 2-14%). Stability of secondary metabolism of F. officinalis
and F. thuretii indicates for genetic stability of Bulgarian Fumaria species growing in different populations.
populations, naturally grown in Bulgaria has been studied. Significant differences between concentrations of
main polyphenols (rutin, hyperoside and syringic acid) and antioxidant activity in two different populations of
both analyzed species were not established (RSD 2-14%). Stability of secondary metabolism of F. officinalis
and F. thuretii indicates for genetic stability of Bulgarian Fumaria species growing in different populations.
Research Interests:
Fumaria plants have been traditionally used against skin diseases, as diuretics, laxatives, hepatoprotectants and etc. Their biological activity is associated with the presence of isoquinoline alkaloids, while polyphenols are not... more
Fumaria plants have been traditionally used against skin diseases, as diuretics, laxatives, hepatoprotectants and etc. Their biological activity is associated with the presence of isoquinoline alkaloids, while polyphenols are not investigated well in this context. Polyphenols could increase the added value of medicinal plants extracts by enhancing their antioxidant activity or improving overall biological activities. The presented study evaluated the polyphenolic contents and antioxidant activities of extracts of five Bulgarian Fumaria species (Fumariaceae): Fumaria officinalis L., Fumaria thuretii Boiss., Fumaria kralikii Jord., Fumaria rostellata Knaf. and Fumaria schrammii (Asch) Velen. Qualitative and quantitative determinations of phenolic acids and flavonoids were performed by HPLC system with UV-detection. Antioxidant activity of extracts was evaluated by four popular spectrophotometric methods (DPPH, ABTS, FRAP and CUPRAC). The total phenolic content was the highest in extract of F. officinalis with quercetin (0.49 ± 0.03 mg/g DW), p-coumaric (1.10 ± 0.03 mg/g DW) and ferulic (2.35 ± 0.04 mg/g DW) acids as major compounds. Theses extracts also showed the highest antioxidant activity among the investigated plants. Our data reveal new possibilities to expand applications of Fumaria extracts from a local ethnomedicine drug to new industrial fields such as foods and cosmetics preservation.
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Ficus carica L. (fig) belongs to mulberry tree (Moraceae) which is one of the oldest fruits in the world. The extracts from fig possess anthelmintic, anticarcinogenic, purgative, aphrodisiac, anti-inflammatory, expectorant, diuretic,... more
Ficus carica L. (fig) belongs to mulberry tree (Moraceae) which is one of the oldest fruits in the world. The extracts from fig possess anthelmintic, anticarcinogenic, purgative, aphrodisiac, anti-inflammatory, expectorant, diuretic, anti-anxiety (mild sedative) activity. Phytoconstituents as umbelliferone, scopoletin, psoralens, bergapten, xanthotoxin, xanthotoxol, marmesin have been isolated from fig leaves. The current study was conducted to evaluate the total polyphenols, total flavonoids (TF), and to investigate the antioxidant capacities of aqueous, methanolic, ethanolic, acetone and n-hexane extracts prepared from Ficus carica leaves. Total phenolic content (TFC) was determined by using the Folin-Ciocalteau method. The antioxidant capacities were evaluated by DPPH and FRAP spectrophotometric methods. The results indicated that TPC, TF, DPPH and FRAP values were higher in aqueous extract of Ficus carica leaves: 6.6 mg GAE / g DW, 2.1 mg QE / g DW, 59.6 mM TE / g DW (DPPH method) and 53.9 mM TE / g DW (FRAP method), respectively. The results clearly demonstrated that these extracts had antioxidant capacity and it could be find significant correlation between total phenolic content and antioxidant activity. Therefore, Ficus carica leaves were evaluated as a rich source of polyphenols and they possessed high antioxidant properties.
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The purpose of the present study was to investigate the antioxidant activity and fructans content in ethanol and water extracts of roots of elecampane (Inula helenium L.), a Bulgarian medicinal plant. The extraction procedure included 95%... more
The purpose of the present study was to investigate the antioxidant activity and fructans content in ethanol and water extracts of roots of elecampane (Inula helenium L.), a Bulgarian medicinal plant. The extraction procedure included 95% (v/v) ethanol extraction and subsequent water treatment. The antioxidant activity was evaluated by several reliable methods such as DPPH-, ABTS-, FRAP- and CUPRAC-assays, as well as the total phenolic content. In addition, the total fructans and sugar content were determinated by spectrophotomeric, TLC and HPLC-RID methods. The level of fructans in ethanol extracts was 14.1 g / 100 g dry weight, as nystose and 1-kestose were only 0.3 g / 100 g dry weight, and 0.5 g / 100g dry weight, respectively. The absence of fructooligosacharides and sugars in water extracts after the ethanol pretreatment was established. Inulin content was evaluated to be 32 g / 100 g dry weight. The metabolites profile of roots revealed their potential application as radical scavengers due to the presence of polyphenols. Therefore, the root extracts of elecampane could be assumed as a rich source of biologically active substance, in particular dietary fiber with potential prebiotic effect, due to the presence of polysaccharide inulin and fructooligosacharides.
Research Interests: Antioxidants and Inulin
Abstract Ultrasonic fractional extraction of fructooligosaccharides and inulin from Jerusalem artichoke tubers has been carried out. The following conditions as the type of the used solvent- n-propanol, 95% (v/v) ethanol and distilled... more
Abstract Ultrasonic fractional extraction of fructooligosaccharides and inulin from Jerusalem artichoke tubers has been carried out. The following conditions as the type of the used solvent- n-propanol, 95% (v/v) ethanol and distilled water and the duration of the extraction process have been varied. The contents of sugars and fructans in the obtained extracts were determinated by spectrophotometric method based on resorcinol reaction and by TLC analysis. The greatest amounts of fructooligosaccharides and inulin have been extracted by distilled water (46 g/100g d.w.), as each ultrasonic extraction was carried out in triplicate for 30 min. The best solvent used for the separation of a particular fraction fructooligosaccharides consisting of kestose, nystose and fructofuranosylnystose was n-propanol.
The current research is dedicated to following the accumulation of prebiotics inulin and fructooligosaccharides in the roots of Bulgarian medicinal plants common chicory (Cichorium intybus L.), dandelion (Taraxacum officinale Web.) and... more
The current research is dedicated to following the accumulation of prebiotics inulin and fructooligosaccharides in the roots of Bulgarian medicinal plants common chicory (Cichorium intybus L.), dandelion (Taraxacum officinale Web.) and meadow salsify (Tragopogon pratensis L.) in some areas of the Bulgaria for a period of two years. The roots of each plant were subjected to exhausting ethanol and subsequent water extraction. The contents of inulin-type fructans in the obtained extracts have been determined by a spectrophotometric method based on resorcinol reaction and TLC analysis. It has been found that the amounts of fructooligosaccharides and inulin were the highest in roots of the plants collected in the autumn season as in taproots of common chicory reached to 23 g/100 g dw and up to 34 g/100 g d.w in dandelion roots, respectively. The underground parts of the studied medicinal plants collected in the spring have been characterized by very high levels of fructo-oligosaccharides and sugars. Meadow salsify showed relatively constant levels of fructans in its taproots (18 g/100g d.w).
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Research Interests: Tlc, Inulin or Oligofructose, Fos, Skills & Expertise : Spectrophotometry TLC-Optical Scanning Densitometry TLC-Image Analysis TLC-Spectrometry Industrial Wate Recycling & Product Generation FTIR Applied Thin Layer Chromatography Colour Quantification and Analytical Applications, Dandelion, and 2 moreTLC-Densitometery and Chicory
Fatty esters of carbohydrates constitute an interesting group of non-ionic surfactants. These compounds are environmentally friendly, with low toxicity and present interest in agriculture, pharmaceuticals, cosmetic and food industry.... more
Fatty esters of carbohydrates constitute an interesting group of non-ionic surfactants. These compounds are environmentally friendly, with low toxicity and present interest in agriculture, pharmaceuticals, cosmetic and food industry. Inulin and fructooligosaccharides esters were synthesized by reacting these carbohydrates with methyl esters of saturated fatty acids (C12 to С18). The esterification reaction was performed in presence of catalyst NaOMe and absence of solvent. A new method for synthesis with the ultrasonic influence has been developed and applied. The fructooligosaccharides esters and modified inulin have been characterized by measurement of melting point, water activity, thin-layer chromatography (TLC) and FT-IR. A preliminary study of their emulsifying and stabilizing properties was analyzed. The rheological properties, dispersion and emulsion stability of oil-in-water emulsions (O/W) prepared with different concentration of inulin esters as emulsifier were investigated.
Research Interests:
Inulin esters were synthesized by transesterification reaction with methyl esters of three fatty acids (10-undecylenic, lauric and palmitic) under ultrasonic irradiation. The obtained esters were characterized by TLC and IR-FT... more
Inulin esters were synthesized by transesterification reaction with methyl esters of three fatty acids (10-undecylenic,
lauric and palmitic) under ultrasonic irradiation. The obtained esters were characterized by TLC and IR-FT spectroscopy. These inulin esters were examined for potential antimicrobial properties. The results from antibacterial assay showed that 1% inulin laurinate inhibited the growth of some food borne pathogenic bacteria - Escherichia coli, Salmonela and B. cereus. Inulin 10-undecylenate and laurinate were active against Candida albicans and Penicillium. Therefore, the current study revealed the potential of future application of synthesized inulin esters in food, pharmaceutical and cosmetic products as potential antimicrobial agents.
lauric and palmitic) under ultrasonic irradiation. The obtained esters were characterized by TLC and IR-FT spectroscopy. These inulin esters were examined for potential antimicrobial properties. The results from antibacterial assay showed that 1% inulin laurinate inhibited the growth of some food borne pathogenic bacteria - Escherichia coli, Salmonela and B. cereus. Inulin 10-undecylenate and laurinate were active against Candida albicans and Penicillium. Therefore, the current study revealed the potential of future application of synthesized inulin esters in food, pharmaceutical and cosmetic products as potential antimicrobial agents.
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The paper presents results obtained by experimental study of polyvinyl chloride (PVC) films containing sucrose palmitate as bio-plasticizer. Influence of the plasticizer’s concentration on morphology and tensile properties of the polymer... more
The paper presents results obtained by experimental study of polyvinyl chloride (PVC) films containing sucrose palmitate as bio-plasticizer. Influence of the plasticizer’s concentration on morphology and tensile properties of the polymer has been investigated in order to prove its plasticizing effect. It has been established a specific nonhomogeneous structure, which changes along with the increase of the plasticizer content. The quantity of the plasticizer influences also the tensile properties of the polymer, whereas their values decrease while it is growing.
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Esters of inulin with different fatty acid (acetic, palmitic and stearic) have been synthesized with intention to their application as additives in food products. The influence of the degree of substitution (DS) on the melting point,... more
Esters of inulin with different fatty acid (acetic, palmitic and stearic) have been synthesized with intention to their application as additives in food products. The influence of the degree of substitution (DS) on the melting point, water activity (aw) and hydrophilic-lipophilic balance (HLB) of synthesized esters were determined.
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The article defined the content of polyuronides and the degree of pectin esterification (DE) in the fruit (peel and seeds) of the two types of rose hips: cultivated and wild rose hips "Plovdiv-1" (harvest 2012). It was found... more
The article defined the content of polyuronides and the degree of pectin esterification (DE) in the fruit (peel and seeds) of the two types of rose hips: cultivated and wild rose hips "Plovdiv-1" (harvest 2012). It was found that the peel contain polyuronides 15,4…16,0 % based on the dry weight and seeds – from 7,3 to 7,5 %. Main part of pectin (about 70%) is found in the skin and has a degree of etherification 67,6…8,4 %. The seeds contain pectin smaller and it has a lower degree of etherification (60 %).
Abstract: The influence of harvest time on fructan (fructooligosaccharides and inulin) content of different varieties of Helianthus tuberosus have been investigated in this study. The determination of quantity of inulin in the tubers... more
Abstract: The influence of harvest time on fructan (fructooligosaccharides and inulin) content of different varieties of Helianthus tuberosus have been investigated in this study. The determination of quantity of inulin in the tubers harvested during three years period from 2010 until 2012 was done. The extraction of fructooligosacharides was carried out with 95 % (v/v) ethanol used as a solvent. Inulin from tubers of Helianthus tuberosus was extracted by treatment with hot distilled water. The extraction efficiency of inulin and fructooligosacharides was followed by TLC analysis. The fructan content in the extracts obtained from the tubers was determined by the spectrophotometric method based on the Seliwanoff reaction with resorcinol and by HPLC-RID method. It was found that a similar amount of inulin and fructooligosacharides (45-53% of dry weight) was observed in the middle early varieties harvested 21-26 weeks after plantation and in the late variety harvested 29-33 weeks after...
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During the current investigation experiments for the preparation of alginate beads with aqueous solutions of sodium alginate, calcium lactate or calcium dichloride and dietary fiber in different concentrations: inulin with varying degrees... more
During the current investigation experiments for the preparation of alginate beads with aqueous solutions of sodium alginate, calcium lactate or calcium dichloride and dietary fiber in different concentrations: inulin with varying degrees of polymerization, wheat bran and amidated apple pectin were carried out. The sodium alginate solutions were at constant concentration 3%, while calcium salts in 7% were applied for bead formation. It was proven that the rupture force of alginate beads was always higher than the pure model system regardless of the chemical structure of dietary fibers used. In the result of the carried research the dependence at a certain concentration was established at which the rupture force and deformation of the beads increased gradually.
Ficus carica L. (fig) belongs to mulberry tree (Moraceae) which is one of the oldest fruits in the world. The extracts from fig possess anthelmintic, anticarcinogenic, purgative, aphrodisiac, anti-inflammatory, expectorant, diuretic,... more
Ficus carica L. (fig) belongs to mulberry tree (Moraceae) which is one of the oldest fruits in the world. The extracts from fig possess anthelmintic, anticarcinogenic, purgative, aphrodisiac, anti-inflammatory, expectorant, diuretic, anti-anxiety (mild sedative) activity. Phytoconstituents as umbelliferone, scopoletin, psoralens, bergapten, xanthotoxin, xanthotoxol, marmesin have been isolated from fig leaves. The current study was conducted to evaluate the total polyphenols, total flavonoids (TF), and to investigate the antioxidant capacities of aqueous, methanolic, ethanolic, acetone and n-hexane extracts prepared from Ficus carica leaves. Total phenolic content (TFC) was determined by using the Folin-Ciocalteau method. The antioxidant capacities were evaluated by DPPH and FRAP spectrophotometric methods. The results indicated that TPC, TF, DPPH and FRAP values were higher in aqueous extract of Ficus carica leaves: 6.6 mg GAE / g DW, 2.1 mg QE / g DW, 59.6 mM TE / g DW (DPPH metho...
Research Interests:
A simple and reproducible high-performance liquid chromatography (HPLC) with refractive index (RI) method for the qualitative and quantitative analysis of five mono-and disaccharides (fructose, glucose, sucrose, maltose and lactose) in... more
A simple and reproducible high-performance liquid chromatography (HPLC) with refractive index (RI) method for the qualitative and quantitative analysis of five mono-and disaccharides (fructose, glucose, sucrose, maltose and lactose) in food products has been developed and validated. The best separation of sugars was achieved with mobile phase acetonitile-water (80:20) and flow rate 2 ml/min. HPLC method showed good linearity with determination coefficients exceeding 0.998. The limits of detection (DL) in these sugars were 0.13, 0.13, 0.21, 0.26 and 0.36 mg/ml, respectively; and the limits of quantification (QL) -0.44, 0.47, 0.51, 0.69 and 0.70 mg/ml. Recoveries in all sugars were between 81 and 121%.
The current research is dedicated to following the accumulation of prebiotics inulin and fructooligosaccharides in the roots of Bulgarian medicinal plants common chicory (Cichorium intybus L.), dandelion (Taraxacum officinale Web.) and... more
The current research is dedicated to following the accumulation of prebiotics inulin and fructooligosaccharides in the roots of Bulgarian medicinal plants common chicory (Cichorium intybus L.), dandelion (Taraxacum officinale Web.) and meadow salsify (Tragopogon pratensis L.) in some areas of the Bulgaria for a period of two years. The roots of each plant were subjected to exhausting ethanol and subsequent water extraction. The contents of inulin-type fructans in the obtained extracts have been determined by a spectrophotometric method based on resorcinol reaction and TLC analysis. It has been found that the amounts of fructooligosaccharides and inulin were the highest in roots of the plants collected in the autumn season as in taproots of common chicory reached to 23 g/100 g dw and up to 34 g/100 g d.w in dandelion roots, respectively. The underground parts of the studied medicinal plants collected in the spring have been characterized by very high levels of fructo-oligosaccharides...
Research Interests:
Polysaccharide inulin has been isolated from the dry taproots of common chicory Cichorium intybus L. by hot water extraction procedure under high-pressure conditions and further precipitation with acetone at low temperature. The obtained... more
Polysaccharide inulin has been isolated from the dry taproots of common chicory Cichorium intybus L. by hot water extraction procedure under high-pressure conditions and further precipitation with acetone at low temperature. The obtained inulin was characterized by the measurement of melting point and angle of optical rotation. The total fructose content was determined by resorcinol spectrophotometric method. The purity of isolated polysaccharide was defined by HPLC-RID method for analysis of inulin. The reducing groups were evaluated by PAHBAH. The protein content was analyzed by Bradford protein assay. IR-FT spectrum was recorded in KBr pellets and the absorption was reported in wavenumbers (cm-1). The isolated substance from chicory roots presented a white powder, without taste. It characterized with the melting point 165 °C, the angle of optical rotation (c=0.5; H2O) and gave positive Selivanoff reaction, an indication for the presence of ketose groups. This carbohydrate was cha...
Abstract Ultrasonic fractional extraction of fructooligosaccharides and inulin from Jerusalem artichoke tubers has been carried out. The following conditions as the type of the used solvent- n-propanol, 95% (v/v) ethanol and distilled... more
Abstract Ultrasonic fractional extraction of fructooligosaccharides and inulin from Jerusalem artichoke tubers has been carried out. The following conditions as the type of the used solvent- n-propanol, 95% (v/v) ethanol and distilled water and the duration of the extraction process have been varied. The contents of sugars and fructans in the obtained extracts were determinated by spectrophotometric method based on resorcinol reaction and by TLC analysis. The greatest amounts of fructooligosaccharides and inulin have been extracted by distilled water (46 g/100g d.w.), as each ultrasonic extraction was carried out in triplicate for 30 min. The best solvent used for the separation of a particular fraction fructooligosaccharides consisting of kestose, nystose and fructofuranosylnystose was n-propanol.
Синтезированных инулин эфиры были охарактеризованы с помощью ИК-спектроскопии, температура плавления, активностью воды (аw) и степень этерификации эфиров инулин. Вода активность синтезированных эфиров инулин ацетат определяется EEJA-3/BAG... more
Синтезированных инулин эфиры были охарактеризованы с помощью ИК-спектроскопии, температура плавления, активностью воды (аw) и степень этерификации эфиров инулин. Вода активность синтезированных эфиров инулин ацетат определяется EEJA-3/BAG Novasina. Температура плавления синтезированных инулин ацетата были измерены на приборе температуры плавления Buchi 510 в капиллярной трубке стекла. степень этерификации. Время ацетилирования составляло 1, 2, 3 5, и 8 часов, в результате реакции получали инулин эфиры (СЗ=0.24; 0.28; 0.33, 0.55). Синтезированные эфиры инулин имеют aw = 0.200 и 0.269, температура плавления: 127-135 °С. Для определения структуры полученных полимеров использованы методы ИК спектроскопии. ИК-спектры регистрировали на ИК-спектрометре Nicolet FT-IR (Avatar Nicolet, TermoScience) в таблетках бромида калия в инфракрасной области 4000–500 см–1. В ИК-спектре выявлены полосы поглощения, характерные для фруктанов типа инулина (ν: 1427, 1332, 1250, 1245, 1134, 1087, 1071, 1031, ...
A rapid and sensitive spectrophotometric method for the quantitative determination of inulin and fructooligosacharides in lactic acid fermented dairy products has been developed. The method was based on the familiar Seliwanoff reaction... more
A rapid and sensitive spectrophotometric method for the quantitative determination of inulin and fructooligosacharides in lactic acid fermented dairy products has been developed. The method was based on the familiar Seliwanoff reaction for detection of ketoses with resorcinol in hydrochloric acid media. The standard curve was constructed by using fructose as a standard. The tested spectrophotometric method has been characterized with good linearity (R2=0.997) in the fructose concentration range 0.5-20 μg/ml. The presence of galactose and lactose in the analyzed samples did not interfere in the determination of inulin. The sample preparation procedure included hot water extraction, purification by Carrez solutions and subsequent spectrophotometric determination at 480 nm. The developed method showed very good precision: repeatability (RSD=6%) and reproducibility (RSD=10%). The obtained results demonstrated that the spectrophotometric method is less time-consuming, cheap, reproducible and suitable for routine laboratory analysis.
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Esters are widely used in in-a-kind and are widely used in industry. The rapid increase in demand for esters in the food industry, cosmetics, pharmaceutical industry makes it imperative to find alternative ways of synthesis instead of... more
Esters are widely used in in-a-kind and are widely used in industry. The rapid increase in demand for esters in the food industry, cosmetics, pharmaceutical industry makes it imperative to find alternative ways of synthesis instead of reactions that have low yield, expensive, energy-intensive and long lasting. Also, long-chain esters of higher fatty acids are used in industry as biodiesel. The Production process for the synthesis of esters is based on the chemical esterification of fatty acids with an alcohol in the presence of inorganic catalysts at high temperatures. However, these chemical processes are non-selective and consume large amount of energy. In this connection, the current situation of environmental degradation requires the development of efficient and environmentally friendly methods in terms of energy consumption and reduce waste. The synthesis of esters by means of ultrasound is also economical and efficient method for carrying out the esterification with wide pract...
Fatty esters of carbohydrates constitute an interesting group of non-ionic surfactants. These compounds are environmentally friendly, with low toxicity and present interest in agriculture, pharmaceuticals, cosmetic and food industry.... more
Fatty esters of carbohydrates constitute an interesting group of non-ionic surfactants. These compounds are environmentally friendly, with low toxicity and present interest in agriculture, pharmaceuticals, cosmetic and food industry. Inulin and fructooligosaccharides esters were synthesized by reacting these carbohydrates with methyl esters of saturated fatty acids (C12 to С18). The esterification reaction was performed in presence of catalyst NaOMe and absence of solvent. A new method for synthesis with the ultrasonic influence has been developed and applied. The fructooligosaccharides esters and modified inulin have been characterized by measurement of melting point, water activity, thin-layer chromatography (TLC) and FT-IR. A preliminary study of their emulsifying and stabilizing properties was analyzed. The rheological properties, dispersion and emulsion stability of oil-in-water emulsions (O/W) prepared with different concentration of inulin esters as emulsifier were investigated.
Research Interests:
The aim of the current study was to investigate the carbohydrate composition, total phenolic content and in vitro antioxidant activities of 70 % (v/v) ethanol extracts obtained from fruits and leaves of three Morus species: white mulberry... more
The aim of the current study was to investigate the carbohydrate composition, total phenolic content and in vitro antioxidant activities of 70 % (v/v) ethanol extracts obtained from fruits and leaves of three Morus species: white mulberry (Morus alba), black mulberry (Morus nigra) and red mulberry (Morus rubra) grown in Bulgaria. The carbohydrate content was determinated by spectrophotomeric, TLC and HPLC-RID methods. The total phenolic content was analyzed by Folin–Ciocalteau method. The antioxidant activities of above mentioned extracts were evaluated by DPPH and FRAP assays. From the obtained results, monosaccharides fructose and glucose were found to be the main sugars in all investigated extracts, as their content reached to 3.0 g/100 g fw in fruits and 0.6 g/100g fw in leaves. M. alba and M. rubra fruits were evaluated as a natural source of prebiotic, due to the presence of 1-kestose, nystose and inulin. The absence of sucrose in all mulberry fruit extracts was also establish...
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The production of functional lactic acid dairy products constantly increases nowadays. They are usually used to enrich with soluble dietary fibers that enhance their functional characteristics. The aim of the current research was to... more
The production of functional lactic acid dairy products constantly increases nowadays. They are usually used to enrich with soluble dietary fibers that enhance their functional characteristics. The aim of the current research was to investigate the lactic acid dairy products enriched with inulin-type fructans with different degree of polymerization and to analyse the fructooligosacharides and inulin content in them after lactic acid fermentation. For the yoghurt preparation inulin and fructooligosacharides extracted from tubers of Helianthus tuberosus L. and commercially available inulin from chicory were used. The amount of fructans from inulin-type was determined by spectrophotometric, TLC and HPLC-RID methods. The results from the analysis showed that after lactic acid fermentation the content of inulin changed in a very small amount and the obtained product possessed improved healthy and potential prebiotic effect.
The possibilities of preparing stable food emulsions by replacing the milk fat with corn oil in various amounts were studied. For emulsification of corn oil in the milk were used emulsifiers – glycerolmonostearate, soy lecithin and... more
The possibilities of preparing stable food emulsions by replacing the milk fat with corn oil in various amounts were studied. For emulsification of corn oil in the milk were used emulsifiers – glycerolmonostearate, soy lecithin and sunflower lecithin, in concentrations 1g.dm-3. It has been found that the emulsions with lycerolmonostearate by concentration of vegetable oil – 80 cm3.dm-3 and in combination with milkfat - 40 cm3.dm-3 and corn oil - 40 cm3.dm-3 are most stable.
Encapsulation of biologically active substances and their addition in various foods represent great possibilities to obtain products with increased biological value and potential health effects. Encapsulation of chokeberry juice, rich in... more
Encapsulation of biologically active substances and their addition in various foods represent great possibilities to obtain products with increased biological value and potential health effects. Encapsulation of chokeberry juice, rich in polyphenolic compounds and anthocyanins, beetroot extract containing betalain in alginate- inulin beads were used to obtained new functional food product. The prepared fermented dairy dessert, with added alginate-inulin beads with biologically active substances was characterized with very good organoleptic properties and represented a product containing substances with known beneficial effect on human health.
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Wild strawberries Fragaria vesca L. have been traditionally used in herbal medicine in treating rashes, as well as internally for treating gastrointestinal catarrh (mucous), diarrhea, intestinal toning, liver health maintenance, catarrh... more
Wild strawberries Fragaria vesca L. have been traditionally used in herbal medicine in treating rashes, as well as internally for treating gastrointestinal catarrh (mucous), diarrhea, intestinal toning, liver health maintenance, catarrh of respiratory passages, rheumatism, nervousness, bladder health maintenance, gravel, fever, in support of vascular health and as a diuretic. The leaves of Fragaria vesca L. are natural source of biologically active substance, such as condensed tannins (epigallocatechins), ellagitannins (pedunculagin and agrimoniin), flavonoids (kampferol and quercetin glucosides) and proantocyanidins (catechin, procyanidin B1). The aim of the current investigation is connected with selection of the best conditions for proanthocyanidins extraction. The influence of the duration of the ultrasonic extraction and solvent system (acetone-water) in different concentration ratio over the extraction process was studied. The optimal conditions for the extraction of proantocy...
The Jerusalem artichoke (Helianthus tuberosus L.) known also as topinambour is plant with high importance for human and animal nutrition during the last decade. Its tubers were consumed fresh, stewed or they were added as flour in food... more
The Jerusalem artichoke (Helianthus tuberosus L.) known also as topinambour is plant with high importance for human and animal nutrition during the last decade. Its tubers were consumed fresh, stewed or they were added as flour in food products to improve their functional properties. In the current research a comparative study of bioactive substance and free radical scavenging activities of flour obtained from tubers of different varieties and wild populations of Jerusalem artichoke (Helianthus tuberosus L.) grown on territory of Bulgaria was done. The ultrasonic irradiation (42 kHz) was performed with 70 % ethanol and distilled water as solvents to accelerate extraction process. The total fructans, phenolic content and radical scavenging activities of the extracts were investigated. The 70 % ethanol extracts possessed the highest total phenolic content (6-17 mg GAE/g dry weight) and antioxidant activity defined by ABTS and CUPRAC methods. The water extracts characterized with highe...
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A single, selective and rapid HPLC-RID method for determination of inulin, nystose, kestose, sucrose, glucose and fructose in foods has been developed. The best chromatography conditions for separation were obtained on Pb2++ column... more
A single, selective and rapid HPLC-RID method for determination of inulin, nystose, kestose, sucrose, glucose and fructose in foods has been developed. The best chromatography conditions for separation were obtained on Pb2++ column operating at 85oC, mobile phase deionized water and flow rate 1.0 ml/min. The linearity of the method was in the concentration rate 0.1-10 mg/ml for all analyzed carbohydrates with the correlation coefficient R2 > 0.997. The limit of detection (LOD) and the limit of quantification (LOQ) for inulin, nystose, kestose, sucrose, glucose and fructose have also been defined. The developed method showed very good repeatability and reproducibility (RSD 2-3%) and it was succassfully applied to determine inuline, fructooligosaccharides and sugars in functional food products and the medicinal plant root extract.
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The object of our current study is to determinate the biologically active substance presented in the taproots of Bulgarian medicinal plant common chicory (Cichorium intybus L.). The carbohydrate composition, the amount of total phenols,... more
The object of our current study is to determinate the biologically active substance presented in the taproots of Bulgarian medicinal plant common chicory (Cichorium intybus L.). The carbohydrate composition, the amount of total phenols, the total flavanoides content and the antioxidant activity in the obtained sequential ethanol and water extracts has been evaluated. The amount of inulin-type fructans was defined by the resorcinol assay. The sugars, fructooligosacharides and inulin contents of the obtained extracts were analyzed by TLC and HPLC-RID methods. The total phenolic and flavonoid quantities were analysed by using Folin–Ciocalteu's and Al(NO 3) 3 reagents, respectively. The antioxidant activity was defined by four method (DPPH, ABTS, FRAP and CUPRAC). The total fructan content in taproots is in range 23 % dw. The presence of monosaccharide glucose, fructose, sucrose and trisaccharides 1-kestose, nystose in the ethanol extracts was observed in the ethanol extracts. The a...